septin 9 (Proteintech)
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Proteintech
septin 9
Septin 9, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/septin+9/SEPT9+Antibody/pm39503295-193-71-73
Average 92 stars, based on 45 article reviews
Septin 9, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/septin+9/SEPT9+Antibody/pm39503295-193-71-73
Average 92 stars, based on 45 article reviews
septin 9 - by Bioz Stars,
2026-11
92/100 stars
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Immunofluorescence:Article Title: Borg5 restricts contractility and motility in epithelial MDCK cells Article Snippet: .. The following primary antibodies were used for immunofluorescence (IF) and/or immunoblotting (IB): Cdc42EP1/Borg5 (Proteintech, 27904-1-AP, IB 1:500, IF 1:100); Myc tag (clone 9E10, home-made, IB and IF 1:500) or c-Myc tag (Proteintech #16286-1-AP, IF 1:250, IB 1:1000); Vinculin (Novus Biologicals, #NB120-11193, IF 1:100 or Proteintech 26520-1-AP, IF 1:600), ZO1 (clone R26.4C; , 1:50 IF); septin 2 (Proteintech #11397-1-AP, IF 1:300 and #60075-1-Ig, IB 1:1000; BiCell #0022, IF 1:100) Article Title: Borg5 restricts contractility and motility in epithelial MDCK cells. Article Snippet: The Borg (or Cdc42EP) family consists of septin-binding proteins that are known to promote septin-dependent stress fibers and actomyosin contractility.. We show here that epithelial Borg5 (also known as Cdc42EP1) instead limits contractility, cell–cell adhesion tension and motility, as is required for the acquisition of columnar, isotropic cell morphology in mature MDCK monolayers.. Borg5 depletion inhibited the development of the lateral F-actin cortex and stimulated microtubule-dependent leading-edge lamellae as well as radial stress fibers and, independently of the basal F-actin phenotype, caused anisotropy of apical surfaces within compacted monolayers. Western Blot:Article Title: Borg5 restricts contractility and motility in epithelial MDCK cells Article Snippet: .. The following primary antibodies were used for immunofluorescence (IF) and/or immunoblotting (IB): Cdc42EP1/Borg5 (Proteintech, 27904-1-AP, IB 1:500, IF 1:100); Myc tag (clone 9E10, home-made, IB and IF 1:500) or c-Myc tag (Proteintech #16286-1-AP, IF 1:250, IB 1:1000); Vinculin (Novus Biologicals, #NB120-11193, IF 1:100 or Proteintech 26520-1-AP, IF 1:600), ZO1 (clone R26.4C; , 1:50 IF); septin 2 (Proteintech #11397-1-AP, IF 1:300 and #60075-1-Ig, IB 1:1000; BiCell #0022, IF 1:100) Article Title: Borg5 restricts contractility and motility in epithelial MDCK cells. Article Snippet: The Borg (or Cdc42EP) family consists of septin-binding proteins that are known to promote septin-dependent stress fibers and actomyosin contractility.. We show here that epithelial Borg5 (also known as Cdc42EP1) instead limits contractility, cell–cell adhesion tension and motility, as is required for the acquisition of columnar, isotropic cell morphology in mature MDCK monolayers.. Borg5 depletion inhibited the development of the lateral F-actin cortex and stimulated microtubule-dependent leading-edge lamellae as well as radial stress fibers and, independently of the basal F-actin phenotype, caused anisotropy of apical surfaces within compacted monolayers. Plasmid Purification:Article Title: Quantitative and Integrative Proteome Analysis of Peripheral Nerve Myelin Identifies Novel Myelin Proteins and Candidate Neuropathy Loci Article Snippet: .. Antibodies were specific for PLP/DM20 ( Jung et al., 1996 ), plasmolipin ( Bosse et al., 2003 ), myelin and lymphocyte protein (MAL) ( Schaeren-Wiemers et al., 2004 ), PMP22 (ab15506, Abcam), CD9 (PharMingen), CD81 (PharMingen), CMTM5 (CKLF-like MARVEL-transmembrane domain-containing protein 5, also termed PETA-3; ab88964, Abcam; or M-13, Santa Cruz Biotechnology, yielding similar results), CD151 [455807, R&D Systems ( Cowin et al., 2006 )], Immuno-Electron Microscopy:Article Title: Quantitative and Integrative Proteome Analysis of Peripheral Nerve Myelin Identifies Novel Myelin Proteins and Candidate Neuropathy Loci Article Snippet: .. Antibodies were specific for PLP/DM20 ( Jung et al., 1996 ), plasmolipin ( Bosse et al., 2003 ), myelin and lymphocyte protein (MAL) ( Schaeren-Wiemers et al., 2004 ), PMP22 (ab15506, Abcam), CD9 (PharMingen), CD81 (PharMingen), CMTM5 (CKLF-like MARVEL-transmembrane domain-containing protein 5, also termed PETA-3; ab88964, Abcam; or M-13, Santa Cruz Biotechnology, yielding similar results), CD151 [455807, R&D Systems ( Cowin et al., 2006 )], other:Article Title: BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer. Article Snippet: Article Title: Quantitative and Integrative Proteome Analysis of Peripheral Nerve Myelin Identifies Novel Myelin Proteins and Candidate Neuropathy Loci Article Snippet: Raw image files were processed using Affymetrix GCOS 1.3 software to calculate individual probe cell intensity data and generate CEL Data Files. |
SEPT7 immunostaining (red) in BEAS-2B cells infected with P. aeruginosa for 3 h [multiplicity of infection (MOI) =20] (green). Nuclei are labelled with DAPI (cyan). (D) Western blot of SEPT7 and reference protein glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in BEAS-2B cells after infecting with PAK (MOI = 5) for 3 h or not infected (NI) (representative of n= 3 experiments). " width="250" height="auto" />![Fig. 2. SEPT7 mRNA expression analysis using qPCR (A) and SEPT7 (B, C), SEPT2 (B, D), SEPT6 (B, D), <t>SEPT9</t> (B, D), SEPT11 (B, D) protein expression using western blot in BEAS-2B after 72 h transfection of 5 nM control siRNA (siCTL, white circles) or SEPT7 siRNA (blue circles) (n=4, *p<0.05, Mann–Whitney test). (E) Amount of intracellular P. aeruginosa (Pa) (CFU/mL) in BEAS-2B cells transfected for 72 h with 5 nM of siCTL or SEPT7 siRNA and then infected for 30 min (orange circles, n=6), 1 h (purple circles, n=5) and 3 h (red circles, n=10) with Pa (MOI=5) [(*p<0.05, analysis of variance (ANOVA), Bonferroni correction)]. (F) IL-6 production by BEAS-2B cells transfected for 72 h with 5 nM of siCTL or SEPT7 siRNA in the non-infected (-, white circles) condition and after 3 h of Pa (red cir cles) infection (MOI=5) [(n= 9; *p<0.05, **p<0.01, ****p<0.0001 analysis of variance (ANOVA), Bonferroni correction)].](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_6185/pm38636185/pm38636185__page4_image1.jpg)
![Fig. 4. (A) Kinetics of SEPT mRNA expression measured by RNAseq in primary bronchial epithelial cells from patients without CF (Non-CF, N = 4) or with CF (N = 4) and infected with P. aeruginosa (PAK strain, MOI 0.25) for 0, 2, 4, or 6 h (*p<0.05: CF vs. non-CF; #p<0.05: CF 4 h vs. CF 2 h, Benjamini and Hochberg corrected p- value) [data were extracted from (Balloy et al., 2015)]. (B) Western blot and quantification of SEPT7 and reference protein GAPDH expression in primary bronchial epithelial cells from non-CF donors (non-CF1, non-CF2) and CF patients (CF1, CF2) at baseline and (C) from non-CF3 and CF2 after 3 h of Pa infection (red circled) (MOI = 10) or non-infected (-; white circle) (n=4; ns: non-significant Mann–Whitney test). (D) Immunostaining of SEPT7 (red) in primary bronchial epithelial cells from non-CF1 and CF1 infected for 3 h with Pa (MOI=20) (green). Nuclei are labelled with DAPI (cyan). (E) Western blot of SEPT7 and reference protein GAPDH in Non-CF2 and CF2 cells after 96 h transfection with 10 nM siCTL or SEPT7 siRNA. Amount of intracellular Pa (CFU/mL) in primary non-CF2 and CF2 cells (n=6) transfected for 72 h with 10 nM CTL (white circle) or SEPT7 (red circles) siRNA and then infected for 4 h with Pa (MOI=10) (n=4; ***p<0.001, ANOVA, Bonferroni correction). (F) Interleukin-6 (IL-6) production by non-CF2 and CF2 cells transfected for 72 h with 10 nM CTL (white circle) or SEPT7 (red circle) siRNA in non-infected and after 4 h of Pa infection (MOI=10) (n= 4; **p<0.01, ***p<0.001, ****p<0.0001, ANOVA, Bonferroni correction).](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_6185/pm38636185/pm38636185__page7_image1.jpg)